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Image of two-dimensional electrophoresis analysis in vehicle + middle cerebral artery occlusion (MCAO), retinoic acid (RA) + MCAO, vehicle + sham, and RA + sham animals ( n =5 per <t>group).</t> <t>Isoelectric</t> focusing was performed at pH 4–7 <t>IPG</t> strips and electrophoresed on 7.5–17.5% gradient SDS gels. Squares indicate the significantly changed protein spots between vehicle + MCAO and RA + MCAO animals.
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Image of two-dimensional electrophoresis analysis in vehicle + middle cerebral artery occlusion (MCAO), retinoic acid (RA) + MCAO, vehicle + sham, and RA + sham animals ( n =5 per <t>group).</t> <t>Isoelectric</t> focusing was performed at pH 4–7 <t>IPG</t> strips and electrophoresed on 7.5–17.5% gradient SDS gels. Squares indicate the significantly changed protein spots between vehicle + MCAO and RA + MCAO animals.
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Image of two-dimensional electrophoresis analysis in vehicle + middle cerebral artery occlusion (MCAO), retinoic acid (RA) + MCAO, vehicle + sham, and RA + sham animals ( n =5 per group). Isoelectric focusing was performed at pH 4–7 IPG strips and electrophoresed on 7.5–17.5% gradient SDS gels. Squares indicate the significantly changed protein spots between vehicle + MCAO and RA + MCAO animals.

Journal: The Journal of Veterinary Medical Science

Article Title: Identification of changed proteins by retinoic acid in cerebral ischemic damage: a proteomic study

doi: 10.1292/jvms.22-0119

Figure Lengend Snippet: Image of two-dimensional electrophoresis analysis in vehicle + middle cerebral artery occlusion (MCAO), retinoic acid (RA) + MCAO, vehicle + sham, and RA + sham animals ( n =5 per group). Isoelectric focusing was performed at pH 4–7 IPG strips and electrophoresed on 7.5–17.5% gradient SDS gels. Squares indicate the significantly changed protein spots between vehicle + MCAO and RA + MCAO animals.

Article Snippet: Isoelectric focusing (IEF) was performed using immobilized pH gradient (IPG) gel strip (pH 4–7, 17 cm, Bio-Rad).

Techniques: Electrophoresis